anti ifitm1 (Cell Signaling Technology Inc)
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Cell Signaling Technology Inc
anti ifitm1
Anti Ifitm1, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+ifitm1/IFITM1+Rabbit+mAb/bio_rxiv__2025__11__17__688928-204-21-23
Average 94 stars, based on 2 article reviews
Anti Ifitm1, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+ifitm1/IFITM1+Rabbit+mAb/bio_rxiv__2025__11__17__688928-204-21-23
Average 94 stars, based on 2 article reviews
anti ifitm1 - by Bioz Stars,
2026-10
94/100 stars
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Staining:Article Title: Opposing activities of IFITM proteins in SARS‐CoV‐2 infection Article Snippet: Cells were infected with SARS‐CoV‐2 for 24 h, after which cells were fixed with 4% paraformaldehyde (Thermo Scientific) for 1 h at room temperature, permeabilized with PBS containing 0.1% Triton X‐100, and blocked with PBS/2% FBS. .. Cells were then stained with mouse‐produced anti‐SARS‐CoV‐2 N (Sino Biological, 40143‐MM08) and a cocktail of rabbit‐produced anti‐IFITM2/3 (ProteinTech, 11714‐1‐AP) and rabbit‐produced Article Title: IFIT3 controls IFIT1 accumulation and specificity preventing self mRNA targeting during the innate immune response Article Snippet: .. For the effect in endogenous ISG15 or IFITM1 expression experiments, cells were stained with anti-ISG15 antibody (Proteintech, #15981-1-AP, diluted 1:100) or Article Title: Opposing activities of IFITM proteins in SARS-CoV-2 infection Article Snippet: Cells were infected with SARS-CoV-2 for 24 hours, after which cells were fixed with 4% paraformaldehyde (Thermo Scientific) for 1 h at room temperature, permeabilized with PBS containing 0.1% TritonX-100, and blocked with PBS/2% FBS. .. Cells were then stained with mouse-produced anti-SARS-CoV-2 N (Sino Biological) and a cocktail of rabbit-produced anti-IFITM2/3 (ProteinTech) and other:Article Title: Prefoldin complex promotes interferon-stimulated gene expression and is inhibited by rotavirus VP3 Article Snippet: Antibodies used in the study included the following: rabbit anti-PFDN3 (A305-403A, Bethyl Laboratories, USA); rabbit anti-PFDN4 (16045-1-AP, Proteintech, China); mouse anti–Flag (F1804, Sigma-Aldrich, USA); Article Title: Prefoldin complex promotes interferon-stimulated gene expression and is inhibited by rotavirus VP3. Article Snippet: Antibodies used in the study included the following: rabbit anti-PFDN3 (A305-403A, Bethyl Laboratories, USA); rabbit anti-PFDN4 (16045-1-AP, Proteintech, China); mouse anti–Flag (F1804, Sigma-Aldrich, USA); mouse anti–Myc (2276S, Cell Signaling Technology, USA); rabbit anti–GAPDH (2118S, Cell Signaling Technology, USA); mouse anti–β actin (4970S, Cell Signaling Technology, USA); rabbit anti–OAS3 (PA5-59539, Invitrogen, USA); rabbit Article Title: The RNA helicase DHX16 recognizes specific viral RNA to trigger RIG-I-dependent innate antiviral immunity Article Snippet: Transient transfections of DNA were performed with TransIT-LT1 (Mirus Bio), Lipofectamine 2000, and Lipofectamine 3000 (Invitrogen) according to the manufacture’s guidelines. Ubiquitin Proteomics:Article Title: The RNA helicase DHX16 recognizes specific viral RNA to trigger RIG-I-dependent innate antiviral immunity Article Snippet: Proteins were visualized using ECL or SuperSignal West Femto chemiluminescence reagents (Pierce) and detected by autoradiography. .. The following primary antibodies were used: anti-DHX16 (1:2000 A301-537A Bethyl), anti-TRIM6 N-term (1:1000 SAB1306751 Sigma), anti-K48-linked Ubiquitin (1:1000 05-1307 Millipore), anti-K63-linked Ubiquitin (1:1000 05-1313 Millipore), anti-Ubiquitin (1:1000 P4D1 Enzo), anti-FLAG (1:2000 F7425 Sigma), anti-HA (1:2000 H6908 Sigma), anti-His (1:2000 H1029 Sigma), anti-Lamin A/C (1:1000 sc-376248 SCBT), anti-RIG-I 1C3 (1:1000 kindly provided by Adolfo Garcia-Sastre: Icahn School of Medicine at Mount Sinai), anti-pIRF3 (S386) (1:1000 ab76493 Abcam), anti-IRF3 (1:1000 H00003661-B01 Abnova), anti-pTBK1 (S172) (1:1000 3300-1 Abcam), anti-TBK1 (1:1000 NB100-56705 Novus), anti-IRF7 (1:1000 4920S CST), Article Title: The RNA helicase DHX16 recognizes specific viral RNA to trigger RIG-I-dependent innate antiviral immunity. Article Snippet: DHX16mutants were generated by site-directedmutagenesis PCR and cloned in-frame into the pCAGGS or pCMV6-Entry (OriGene) expression plasmids using primers listed in Table S1. .. The following primary antibodies were used: anti-DHX16 (1:2000 A301-537A Bethyl), anti-TRIM6 N-term (1:1000 SAB1306751 Sigma), anti-K48-linked Ubiquitin (1:1000 05-1307 Millipore), antiK63-linked Ubiquitin (1:1000 05-1313 Millipore), anti-Ubiquitin (1:1000 P4D1 Enzo), anti-FLAG (1:2000 F7425 Sigma), anti-HA (1:2000 H6908 Sigma), anti-His (1:2000 H1029 Sigma), anti-Lamin A/C (1:1000 sc-376248 SCBT), anti-RIG-I 1C3 (1:1000 kindly provided by Adolfo Garcia-Sastre: Icahn School of Medicine at Mount Sinai), anti-pIRF3 (S386) (1:1000 ab76493 Abcam), anti-IRF3 (1:1000 H00003661-B01 Abnova), anti-pTBK1 (S172) (1:1000 3300-1 Abcam), anti-TBK1 (1:1000 NB100-56705 Novus), anti-IRF7 Cell Reports 38, 110434, March 8, 2022 e5 (1:1000 4920S CST), Expressing:Article Title: IFIT3 controls IFIT1 accumulation and specificity preventing self mRNA targeting during the innate immune response Article Snippet: .. For the effect in endogenous ISG15 or IFITM1 expression experiments, cells were stained with anti-ISG15 antibody (Proteintech, #15981-1-AP, diluted 1:100) or |
